河北省石家庄市番茄黄化曲叶病毒及其伴随DNAβ的分子鉴定及序列分析Molecular Identification and Sequence Analysis of Tomato Yellow Leaf Curl Virus Wit an Associated DNA Betasatellite in Shijiazhuang,Hebei
韩旭良,康忱,田哲娟,王鹏,李亚栋,赵雪芳,闫洪波,刘美霞,吴志明
摘要(Abstract):
在河北石家庄市的番茄上分离得到番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)及其伴随卫星DNA (Betasatellite DNA,又称DNAβ),对其进行分子鉴定及序列分析,明确TYLCV石家庄分离物及其伴随DNAβ分子结构特征及其进化起源。根据已报道的TYLCV及其伴随DNAβ序列设计引物,分别扩增TYLCV石家庄分离物及其伴随DNAβ的全长序列并测序。在NCBI上选取国内外有代表性的TYLCV序列和DNAβ序列,使用MEGA 6.0软件和DNAMAN软件对石家庄TYLCV分离物及其伴随DNAβ进行遗传变异分析。结果表明:TYLCV石家庄分离物全长为2 781 bp,共有6个ORFs,将该分离物定名为TYLCV-SJZ2021。基因组序列比对发现,TYLCV-SJZ2021与山东省的分离物同源性最高(99%);与2015年本实验室报道的TYLCV石家庄分离物相似度为98.67%,存在37处碱基差异,均属于TYLCV以色列株系。TYLCV-SJZ2021的伴随DNAβ全长1 343 bp,其上有病毒在细胞间移动相关蛋白的BC1编码区,序列比对发现该DNAβ与河南TYLCV伴随DNAβ分子最为相似(98%)。从分子水平上鉴定了TYLCV-SJZ2021及其伴随DNAβ的分类地位,为防治该病毒病害及其抗病育种奠定了基础。
关键词(KeyWords): 番茄黄化曲叶病毒;DNAβ;分子鉴定;序列分析
基金项目(Foundation): 河北省重点研发计划项目(19226342D);; 河北省农林科学院创新工程项目(2022KJCXZX-JZS-1)
作者(Author): 韩旭良,康忱,田哲娟,王鹏,李亚栋,赵雪芳,闫洪波,刘美霞,吴志明
参考文献(References):
- [1] Harrison B D,Robinson D J. Natural genomic and antigenic variation in whitefly-transmitted geminiviruses(begomoviruses)[J]. Annual Review of Phytopathology,1999,37(1):369-398.
- [2] Zhou X, Liu Y, Robinson D J, Harrison B D. Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra[J]. The Journal of General Virology,1998,79(4):915-923.
- [3] Shahid Mansoor,Yusuf Zafar,Rob W Briddon. Geminivirus disease complexes:The threat is spreading[J].Trends in Plant Science,2006,11(5):209-212.
- [4] Gupta Neha,Reddy Kishorekumar,Bhattacharyya Dhriti,Chakraborty Supriya. Plant responses to geminivirus infection:Guardians of the plant immunity[J]. Virology Journal,2021,18(1):143.
- [5]张辉.侵染我国番茄双生病毒种类鉴定及致病性分析[D].杭州:浙江大学,2009.
- [6]黄狮,纠敏,李萌,汪伦记,彭红,李净净,王浩权.河南省番茄黄化曲叶病毒的分子鉴定及序列分析[J].河南农业科学,2014,43(7):85-89.
- [7] Adams M J,King A M Q,Carstens E B. Ratification vote on taxonomic proposals to the International Committee on Taxonomy of Viruses(2013)[J]. Archives of Virology,2013,158(9):2023-2030.
- [8] Ferreira M A,Teixeira R M,Fontes E P B. GeminivirusHost Interactions:Action and Reaction in Receptor-Mediated Antiviral Immunity[J]. Viruses,2021,13(5):840.
- [9] Bhattacharyya Dhriti,Gnanasekaran Prabu,Kumar Reddy Kishore, Kushwaha Nirbhay Kumar, Sharma Veerendra Kumar, Yusuf Mohd Aslam, Chakraborty Supriya. A geminivirus betasatellite damages the structural and functional integrity of chloroplasts leading to symptom formation and inhibition of photosynthesis[J]. Journal of Experimental Botany,2015,66(19):5881-5895.
- [10]王浩权,纠敏,汪伦记,邱智军,黄狮,张敏.侵染河南省番茄的番茄黄化曲叶病毒及伴随卫星DNA分子的基因组特征[J].河南农业科学,2016,45(10):71-75+84.
- [11] Lazarowitz G S,Shepherd R J. Geminiviruses:Genome structure and gene function[J]. Critical Reviews in Plant Sciences,1992,11(4):327-349.
- [12] Ito Takuya,Kimbara Junji,Sharma Pradeep,Ikegami Masato. Interaction of tomato yellow leaf curl virus with diverse betasatellites enhances symptom severity[J].Archives of Virology,2009,154(8):1233-1239.
- [13] Gnanasekaran P,Kishorekumar R,Bhattacharyya D,Vinoth K R,Chakraborty S. Multifaceted role of geminivirus associated betasatellite in pathogenesis[J].Molecular Plant Pathology,2019,20(7):1019-1033.
- [14] Briddon R W,Brown J K,Moriones E,Stanley J,Zerbini M,Zhou X. Recommendations for the classification and nomenclature of the DNA-β satellites of begomoviruses[J]. Archives of Virology, 2008, 153(4):763-781.
- [15] Xueping Zhou, Yan Xie, Xiaorong Tao, Zhongkai Zhang,Zhenghe Li,Claude M Fauquet. Characterization of DNAβ associated with bego moviruses in China and evidence for co-evolution with their cognate viral DNAAFN1[J]. Journal of General virology,2003,84(1):237-247.
- [16] Mubin Muhammad,Ijaz Sehrish,Nahid Nazia,Hassan Muhammad, Younus Ayesha, Qazi Javaria, Nawaz-UlRehman Muhammad Shah. Journey of begomovirus betasatellite molecules:From satellites to indispensable partners[J]. Virus Genes,2020,56(1):16-26.
- [17] Medina-Puche L,Orílio A F,Zerbini F M,LozanoDurán R. Small but mighty:Functional landscape of the versatile geminivirus-encoded C4 protein[J]. PLoS Pathogens,2021,17(10):e1009915.
- [18] Singh A K,Chattopadhyay B,Chakraborty S. Biology and interactions of two distinct monopartite begomoviruses and betasatellites associated with radish leaf curl disease in India[J]. Virology Journal,2012,9(1):43.
- [19]彭燕,谢艳,张仲凯,周雪平.侵染稀硷的中国番茄黄化曲叶病毒及其卫星DNA全基因组结构特征[J].微生物学报,2004(1):29-33.
- [20]谢艳,周雪平,李正和,李桂新,张仲凯.与烟草曲叶病毒伴随的新型DNA分子鉴定[J].科学通报,2002(10):768-771.
- [21]白晓娟,李亚栋,王国华,尹庆珍,耿保进,董文琦,周春江,吴志明.番茄黄化曲叶病毒石家庄分离物的分子鉴定及序列分析[J].园艺学报,2015,42(1):167-173.
- [22] Deng D,Mcgrath F P,Robinson J D,Harrison D B.Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers[J]. Annals of Applied Biology,1994,125(2):327-336.
- [23]田鹏,杜朝,宋建军,仇燕,王琳珊.河北省番茄黄化曲叶病毒的分子鉴定及序列分析[J].中国农学通报,2012,28(7):119-125.
- [24] Dry I B,Krake L R,Rigden J E,Rezaian M A. A novel subviral agent associated with a geminivirus:The first report of a DNA satellite[J]. Proceedings of the National Academy of Sciences of the United States of America,1997,94(13):7088-7093.
- [25] Briddon R W, Brown J K, Moriones E, Stanley J,Zerbini M, Zhou X, Fauquet C M. Recommendations for the classification and nomenclature of the DNA-beta satellites of begomoviruses[J]. Archives of Virology,2008,153(4):763-781.
- [26] Jiao X Y,Gong H R,Liu X J,Xie Y,Zhou X P. Etiology of Ageratum yellow vein diseases in South China[J]. Plant Disease,2013,97(11):1497-1503.
- [27] Saeed Muhammad,Behjatnia S A Akbar,Mansoor Shahid,Zafar Yusuf,Hasnain Shahida,Rezaian M Ali. A single complementary-sense transcript of a geminiviral DNA βsatellite is determinant of pathogenicity[J]. Molecular Plant-Microbe Interactions,2005,18(1):7-14.
- [28] Qazi J,Amin I,Mansoor S,Iqbal M J,Briddon R W.Contribution of the satellite encoded gene betaC1 to cotton leaf curl disease symptoms[J]. Virus Research,2007,128(1-2):135-139.