韩旭良,康忱,田哲娟,王鹏,李亚栋,赵雪芳,闫洪波,刘美霞,吴志明

• 1:河北经贸大学
• 2:河北省农林科学院经济作物研究所
• 3:山东省寿光市圣城街道办事处

摘要(Abstract)：

在河北石家庄市的番茄上分离得到番茄黄化曲叶病毒（tomato yellow leaf curl virus,TYLCV）及其伴随卫星DNA (Betasatellite DNA，又称DNAβ)，对其进行分子鉴定及序列分析，明确TYLCV石家庄分离物及其伴随DNAβ分子结构特征及其进化起源。根据已报道的TYLCV及其伴随DNAβ序列设计引物，分别扩增TYLCV石家庄分离物及其伴随DNAβ的全长序列并测序。在NCBI上选取国内外有代表性的TYLCV序列和DNAβ序列，使用MEGA 6.0软件和DNAMAN软件对石家庄TYLCV分离物及其伴随DNAβ进行遗传变异分析。结果表明：TYLCV石家庄分离物全长为2 781 bp，共有6个ORFs，将该分离物定名为TYLCV-SJZ2021。基因组序列比对发现，TYLCV-SJZ2021与山东省的分离物同源性最高（99%）；与2015年本实验室报道的TYLCV石家庄分离物相似度为98.67%，存在37处碱基差异，均属于TYLCV以色列株系。TYLCV-SJZ2021的伴随DNAβ全长1 343 bp，其上有病毒在细胞间移动相关蛋白的BC1编码区，序列比对发现该DNAβ与河南TYLCV伴随DNAβ分子最为相似（98%）。从分子水平上鉴定了TYLCV-SJZ2021及其伴随DNAβ的分类地位，为防治该病毒病害及其抗病育种奠定了基础。

关键词(KeyWords)： 番茄黄化曲叶病毒;DNAβ;分子鉴定;序列分析

Abstract:

Keywords:

基金项目(Foundation): 河北省重点研发计划项目（19226342D）;; 河北省农林科学院创新工程项目（2022KJCXZX-JZS-1）

作者(Author): 韩旭良,康忱,田哲娟,王鹏,李亚栋,赵雪芳,闫洪波,刘美霞,吴志明

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