陈治芳;王文桥;韩秀英;张小风;马志强;

• 1:河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心

摘要(Abstract)：

综合评述了灰霉病菌对苯并咪唑类、二甲酰亚胺类、N-苯基氨基甲酸酯类和苯胺基嘧啶类等杀菌剂的抗性及其抗性机制,并简单介绍了可用于治理灰霉病菌抗药性的吡咯类、酰胺类、吡啶类和甾醇生物合成抑制剂类等新型杀菌剂的生物学特性及其作用机理。

关键词(KeyWords)： 灰霉病;灰葡萄孢;杀菌剂;化学防治;抗药性

Abstract:

Keywords:

基金项目(Foundation): 农业公益性行业科研专项(200903033)

作者(Author): 陈治芳;王文桥;韩秀英;张小风;马志强;

Email:

DOI: 10.16318/j.cnki.hbnykx.2010.08.006

参考文献(References)：

• [1]Williamson B,Tudzunski B,Tudzunski P,et al.Botrytis ci-nerea:the cause of grey mould disease[J].Molecular PlantPathology,2007,(8):561-580.
• [2]王树桐,胡同乐,张凤巧.中药细辛提取物对番茄灰霉病菌的抑菌作用及防病效果研究[J].河北农业大学学报,2006,29:59-62.
• [3]周宇.灰葡萄孢菌株CanBC-3致病力衰退及其机制研究[D].武汉:华中农业大学,2003.
• [4]贾晓华.番茄灰霉病菌和油菜菌核病菌对嘧霉胺的敏感基线及番茄灰霉病菌抗药性研究[D].南京:南京农业大学,2004.
• [5]张从宇,张子学,崔广荣.安徽省番茄灰霉菌抗药性测定和治理[J].植物保护,2006,32(3):32-34.
• [6]靳秀兰.番茄灰霉病及其防治研究[J].平原大学学报,2004,21(3):19-20.
• [7]张英,杨松,宋宝安.苯并咪唑类化合物杀菌活性的研究进展[J].农药,2008,47(3):164-170.
• [8]刘长令.农用杀菌剂开发的新进展[J].农药科学与管理,2000,21(3):20-26.
• [9]Kalamarakis A E,Petsikos-Panagiotarou N,Mavroidis B,etal.Activity of fluazinam against strains of Botrytis cinerea re-sistant to benzimidazoles and/or dicarboximides and to a benz-imidazole-phenylcarbamate mixture[J].Journal of Phytopa-thology,2000,148(7/8):449-455.
• [10]司乃国,张宗俭,刘君丽.创制杀菌剂啶菌噁唑生物活性及应用研究(I)—番茄灰霉病[J].农药,2004,43(1):16-18.
• [11]刘君丽,司乃国,陈亮.创制杀菌剂啶菌噁唑生物活性及应用研究(III)—番茄叶霉病[J].农药,2004,43(3):103-105.
• [12]韩平,刘鹏飞,刘西莉.新型杀菌剂啶菌噁唑对番茄灰霉病菌麦角甾醇生物合成的影响[J].分析化学,2006,34:1467-1470.
• [13]刘茜莉,韩平,张真真,等.啶菌噁唑对番茄灰霉病菌的抑菌作用研究[J].农药学学报,2010,12(1):32-48.
• [14]马建英,马志强,王红刚,等.番茄灰霉病菌菌对啶菌噁唑的敏感性基线及抗药突变体的诱导与生物学性状[J].农药学学报,2009,11(1):141-144.
• [15]Jacometti M A,Wratten S D,Walter M.Management ofunderstorey to reduce the primary inoculum Botrytis cinerea:Enhancing ecosystem services in vineyards[J].BiologicalControl,2007,40:57–64.
• [16]Jacometti M A,Wratten,S D,Walter M.Understoreymanagement increases grape quality,yield and resistance toBotrytis cinerea[J].Agriculture,Ecosystems and Envi-ronment,2007,122:349-356.
• [17]Decognet V,Ravetti F,Martin C,et al.Improved leafpruning reduces development of stem cankers caused by graymould in greenhouse tomatoes[J].Agronomy for Sustain-able Development,2010,30(2):465-472.
• [18]Verhoeff K.Studies on Botrytis cinerea in tomatoes influenceof methods of deleafing on the occurrence of stem lesions[J].European Journal of Plant Pathology,1967,73(4):117-120.
• [19]Jacometti M A,Wratten S D,Walter M.Review:Alterna-tives to synthetic fungicides for Botrytis cinerea managementin vineyards[J].Australian Journal of Grape and WineResearch,2010,16(1):154-172.
• [20]褚长彬,吴淑杭,周德平,等.木霉T68对植物病原菌的拮抗作用及对绿豆插条不定根发生的影响[J].农业环境科学学报,2008,27(3):1084-1089.
• [21]王未名,陈建爱,孙永堂,等.六种土传病原菌被木霉抑制作用机理的初步研究[J].中国生物防治,1999,15(3):142-143.
• [22]陈双臣,刘爱荣,郑继亮,等.放线菌对番茄灰霉病和早疫病的拮抗作用研究[J].植物保护,2008,(9):171-173.
• [23]马林,韩巨才,刘慧平.辣椒内生放线菌LJ20活性代谢物抑菌作用的研究[J].中国生态农业学报,2008,16(3):664-668.
• [24]Todorova S,Kozhuharova L.Characteristics and antimicrobi-al activity of Bacillus subtilis strains isolated from soil[J].World Journal of microbiology&biotechnology,2010,26(7):1207-1216.
• [25]李永刚,宋兴舜,赵雪莹,等.生防枯草芽孢杆菌L1特性的初步研究[J].植物保护,2008,34(1):57-61.
• [26]Saligkarias I D,Gravanis F T,Epton H A S.Biologicalcontrol of Botrytis cinerea on tomato plants by the use of epi-phytic yeasts Candida guilliermondii strains 101 and US 7and Candida oleophila strain I-182:in vivo studies[J].Biological Control,2002,25:143-150.
• [27]Shutong W,Tongle H,Yanling J.Isolation and character-ization of Bacillus subtilis EB-28,an endophytic bacteriumstrain displaying biocontrol activity against Botrytis cinereaPers[J].Frontiers of Agriculture in China,2009,3(3):247-252.
• [28]Todorova S,Kozhuharova L.Characteristics and antimicrobi-al activity of Bacillus subtilis strains isolated from soil[J].World Journal of Microbiology&Biotechnology,2010,26(7):1207-1216.
• [29]Trotel-Aziz P,Couderchet M,Biagianti S,et al.Charac-terization of new bacterial biocontrol agents Acinetobacter,Bacillus,Pantoea and Pseudomonas spp.mediating grape-vine resistance against Botrytis cinerea[J].Environmentaland Experimental Botany,2008,64:21-32.
• [30]Hou J,Gao Y N,Feng J T,et al.Sensitivity of Botrytis ci-nerea to propamidine:in vitro determination of baseline sen-sitivity and the risk of resistance[J].European Journal ofPlant Pathology,2010,128(2):261-267.
• [31]Smith C M.History of benzimidazole use and resistance.Fungicides Resistance in North America[M].St.Paul,Minn:ARS Press,1988:23-25.
• [32]Bollen G J.Acquired resistance to benomyl and some othersystemic fungicides in a stain of Botrytis cinerea in cyclamen[J].Plant Pathology,1971,77:83-90.
• [33]Gullino M L,Garibaldi A.Caracterization of dicarboximide-resistant strains of Botrytis cinerea pers.naturally occurring inItaly[J].Meded Fac.Landbouwwet Rijks Univ Gent,l982,47:781-791.
• [34]Beever R E,O'Flaherty B F.Low-level of benzimidazole re-sistance in Botrytis cinerea in New Zealand[J].New Zeal-and Journal of Agricultural Research,1985,28:289-292.
• [35]周明国,叶钟音.南京市郊灰霉菌对苯并咪唑类杀菌剂田间抗性检测[J].南京农业大学学报,1987,6(2):53-57.
• [36]周明国,叶钟音.植物病原菌对苯并咪唑类及相关杀菌剂的抗药性[J].植物保护,1987,18(2):31-33.
• [37]周明国,叶钟音,刘经芬.杀菌剂抗性研究进展[J].南京农业大学学报,1994,17(3):33-41.
• [38]刘慧平,韩巨才,阎秀琴,等.灰霉病原菌对多菌灵的抗性监测[J].山西农业大学学报,2003,23(2):93-97.
• [39]Northover J.Persistence of dicarboximide resistant Botrytiscinerea in Ontario vineyards[J].Canadian Jourmal ofPlant Pathology,1988,10:123-132.
• [40]石志琦,史建荣,陈怀谷,等.小麦赤霉病菌对多菌灵的抗药性研究[J].农药学学报,2000,2(4):22-27.
• [41]刘波,叶钟音,刘经芬,等.对多菌灵、腐霉利具有抗性的灰霉病菌菌株性质的研究[J].南京农业大学学报,1993,16(3):50-54.
• [42]Faretra F,Anionacei E,Pollastro S.Improvement of thetechnique used for obtaining apothecia of Botryotinia fuckeli-ana(Botrytis cinerea)under controlled conditions[J].Annalidi Microbiologiaed Enzimologia,1988,38:29-40.
• [43]Lamandia J A,Douglas S M.Sensitivity of Botrytis cinereafrom comecticut greenhouses to benzimidazole and dicarbox-imide fungicides[J].Plant Disease,1997,81:729-723.
• [44]Pierre L,RenéF,Danièle D,et al.Mechanisms of resist-ance to fungicides in field strains of Botrytis cinerea[J].Pest Management Science,2002,58(9):876-888.
• [45]周明国,王建新,鞠理红,等.小麦赤霉病对多菌灵抗药性变异研究[J].南京农业大学学报,1994,17(增刊):106-112.
• [46]韩巨才,闰秀琴,刘慧平,等.灰霉病原菌对速克灵的抗性监测[J].山西农业大学学报,2003,23(4):299-302.
• [47]纪兆林,童蕴慧,张建军,等.灰葡萄孢对腐霉利抗性的研究[J].扬州大学学报,2003,24(3):60-63.
• [48]Davis R P,Dennis C.Properties of dicarboximide resistantstrains of Botrytis cinerea[J].Pesticide Science,1981,(12):521-535.
• [49]Fraile A.Some characteristic of Botrytis cinerea isolates toler-ant to procymidone[J].Plant Pathology,1986,35:82-85.
• [50]Hisada Y.Fitness of procymidone resistant Botrystis cinereastrains developed in vitro[J].Plant Pathology,1981,87:243-244.
• [51]刘波,叶钟音,刘经芬,等.灰霉病菌速克灵抗性菌株性质的研究[J].植物保护学报,1992,19(4):297-302.
• [52]Elad Y,Shabi E,Katan T.Negative cross resistance be-tween benzimidazole and N-phenylcarbamate fungicides andcontrol of Botrystis cinerea on grapes[J].Plant Pathology,1988,37:141-147.
• [53]Beever R E,Laraey E P,Park H A.Strain of Botrytis cine-rea resistant to dicarboximide and benzimidaole fungdieidesin New Zealand vineyards[J].Plant Pathology,1989,38:427-37.
• [54]Faretra F,Pollastro S,Di Tonno A P.New natural variantsof Botrystis cinerea coupling benzimidazole-resistant to insen-sitivity toward the N-Phenylearbamate diethofencarb[J].Phytopathologia Mediterranea,1989,28:98-104.
• [55]Katan T,Elad Y,Yunis H.Resistance to diethofencarb(NPC)in benomyl-resistant field isolates of Botrystis cinerea[J].Plant Pathology,1989,38:86-92.
• [56]Leroux P,Lanen C,Fritz R.Similarities in the antifungalactivities of fenpiclonil,iprodione and toclofos-methyl a-gainst Botrystis cinerea and Fusarium nivale[J].PesticideScience,1992,36:255-261.
• [57]纪明山,程根武,张益先,等.灰霉病菌对多菌灵和乙霉威抗性研究[J].沈阳农业大学学报,1998,29(3):213-216.
• [58]丁中,刘峰,慕立义,等.不同抗性型灰葡萄孢菌对不同作用机制杀菌剂的敏感性研究[J].农药学学报,2001,3(4):59-63.
• [59]朱桂宁,黄福新,蔡健,等.广西番茄灰霉病菌的多重抗药性检测[J].中国蔬菜,2003,(4):14-16.
• [60]韩巨才,刘慧平,闰秀琴,等.灰霉病菌对三种杀菌剂的抗性表现型分布及稳定性测定[J].农药学学报,2004,6(3):43-47.
• [61]Dianez F,Santos M,Bjaneo R,et al.Fungicide resistancein Botrystis cinerea isolates from strawberry crops in Huelva(Southwestern Spain)[J].Phytoparasitica,2002,30(5):529-534.
• [62]Latorre B A,Spadaro I,Rioja M E.Occurrence of resistantstrains of Botrystis cinerea to anllinopyrimidine fungicides intable grapes in Chile[J].Crop Protection,2002,21(10):957-961.
• [63]纪明山,祁之秋,王英姿,等.番茄灰霉病菌对嘧霉胺的抗药性[J].植物保护学报,2003,30(4):396-400.
• [64]Myresiotis C K,Karaoglanidis G S,Tzavella-KlonariK.Resistance of Botrytis cinerea isolates from vegetablecrops to anilinopyrimidine,phenylpyrrole,hydroxyani-lide,benzimidazole,and dicarboximide fungicides[J].Plant Disease,2007,91(4):407-413.
• [65]Pierre L,RenéF,Danièle D,et al.Mechanisms of resist-ance to fungicides in field strains of Botrytis cinerea[J].Pest Management Science,2002,58(9):876-888.
• [66]Yarden O,Katan T.Mutation leading to substitutions at ami-noacids 198 and 200 of beta-tubulin that correlated withbenomyl-resistance phenotypes of field strains of Botrytiscinerea[J].Phytopathology,1993,83:1478-1483.
• [67]Oshima M,Fujimural M,Banno S,et al.A point mutationin the two-component histidine kinase BcOS-1 gene confersdicarboximide resistance in field isolates of Botrytis cinerea[J].Phytopathology,2002,92:75-78.
• [68]Wei C,Beever R E,Parkes S L,et al.Evolution of an os-mosensing histidine kinase in field strains of Botryotiniafuckeliana(Botrytis cinerea)in response to dicarboximidefungicide usage[J].Phytopathology,2004,94(10):1129-1135.
• [69]Michiyo O,Shinpei B,Kiyot S O,et al.Survey of muta-tions of a histidine kinase gene BcOS1 in dicarboximide re-sistant field isolates of Botrytis cinerea[J].Plant Patholo-gy,2006,72:65-73.
• [70]王芊.番茄灰霉病菌抗药性及抗药性控制研究[D].哈尔滨:东北林业大学,2007.
• [71]Wang Z N.Dicarboximide resistance in Botrytis cinerea inprotected lettuce[J].Plant Pathology,1986,35:427-433.
• [72]Leroux P,Fritz R,Debieu D,et al.Mechanisms of resist-ance to fungicides in field strains of Botrytis cinerea[J].Pest Management Science,2002,58:876-888.
• [73]朱丽华.田间番茄灰霉病菌菌株对杀菌剂抗性的机理[J].世界农药,2003,25(3):39-44.
• [74]Saito S,Suzuki S,Takayanagi T.Nested PCR-RFLP is ahigh-speed method to detect fungicide-resistant Botrytis cine-rea at an early growth stage of grapes[J].Pest Manage-ment Science,2009,65:197-204.