河北地区蚯蚓种群遗传多样性分析Analysis of Genetic Diversity of Drawida gisti and Soil Properties
张玉峰;刘莹莹;李丛胜;毕艳孟;申磊;
摘要(Abstract):
为了探讨蚯蚓遗传多样性与土壤环境的关系,2015年6~10月在河北地区进行蚯蚓资源调查和样品采集,利用PCR技术扩增线粒体基因16S r DNA、ND1序列,分析了该区蚯蚓优势种类天锡杜拉蚓(Drawida gisti)的遗传多样性。结果表明:来自河北23个采集点的研究样本,16S、ND1全部DNA序列的平均单倍型多样性分别为0.76和0.64,平均核苷酸多样性分别为0.042和0.039,群体间平均遗传距离分别为0.045和0.054。土壤性质显著影响种群的遗传变异水平。
关键词(KeyWords): 蚯蚓;天锡杜拉蚓;遗传多样性;土壤性质;河北地区
基金项目(Foundation): 河北省自然科学基金项目(C2015408038);; 河北省大学生创新创业训练项目(201510100034);; 河北省高等学校科学技术研究项目(QN2014055);; 廊坊师范学院博士自然科学基金项目(LSBS201305)
作者(Author): 张玉峰;刘莹莹;李丛胜;毕艳孟;申磊;
Email:
DOI: 10.16318/j.cnki.hbnykx.2016.04.013
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